DNA gene synthesis

This site all is about gene synthesis. First of all, we need to give definition. Artificial gene synthesis is the process of synthesizing a gene without initial template DNA samples. The main method fot doing this is currently by oligonucleotide synthesis (also used for other applications) from digital genetic sequences and subsequent annealing of the resultant fragments. In contrast to the artificial synthesis, natural DNA replication requires existing DNA templates for synthesizing new DNA.

For the first time synthesis of complete gene was developed by Har Gobind Khorana and his coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.

Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. So, gene synthesis is a popular business activity. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures. The market for gene synthesis was growing constantly over the past years. Estimated volume of this market is up to 40 Mio US-$ by the end of 2007.